Monday, March 10, 2008

Immunohistochemistry suggested to differentiate between yellow fever and dengue

The recent yellow fever epidemic in Paraguay with a number of urban cases highlighted the importance of differentiating between dengue and yellow fever in areas where these two Flaviviruses cocirculate. Dr. C.J. Peters at the University of Texas Medical Branch suggested on ProMedMail to use immunohistochemistry for this purpose. Immunohistochemistry localizes the antigens (virus) of the specific disease by presenting labeled antibodies. This method works in fixed tissues which are much easier to obtain from outbreaks in remote locations than fresh tissue or blood samples. He also lists a number of examples when such a method was shown to be effective for yellow fever.
This method seems to be low-tech and efficient, probably cheap method, which I, coming from Hungary, can appreciate.

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